- Within each sample, the values are independent, and identically normally distributed (same mean and variance).
- The two samples are independent of each other.
- For the usual two-sample t test, the two different samples are assumed to come from populations with the same variance, allowing for a pooled estimate of the variance. However, if the two sample variances are clearly different, a variant test, the Welch-Satterthwaite t test, is used to test whether the means are different.
To properly analyze and interpret results of the two-sample unpaired t test, you should be familiar with the following terms and concepts:
Failure to understand and properly apply two-sample unpaired t tests may result in drawing erroneous conclusions from your data. If you are not familiar with these terms and concepts, you may wish to consult with a statistician. You may also want to consult the following references:
- Brownlee, K. A. 1965. Statistical Theory and Methodology in Science and Engineering. New York: John Wiley & Sons.
- Daniel, Wayne W. 1995. Biostatistics. 6th ed. New York: John Wiley & Sons.
- Miller, Rupert G. Jr. 1996. Beyond ANOVA, Basics of Applied Statistics. 2nd. ed. London: Chapman & Hall.
- Rosner, Bernard. 1995. Fundamentals of Biostatistics. 4th ed. Belmont, California: Duxbury Press.
- Sokal, Robert R. and Rohlf, F. James. 1995. Biometry. 3rd. ed. New York: W. H. Freeman and Co.
- Zar, Jerrold H. 1996. Biostatistical Analysis. 3rd ed. Upper Saddle River, NJ: Prentice-Hall.